Biological Activity of Volatile Di-n-Propyl Disulfide from Seeds of Neem, Azadirachta indica (Meliaceae), to Two Species of Stored Grain Pests, Sitophilus oryzae (L.) and Tribolium castaneum (Herbst)

2004 ◽  
Vol 97 (3) ◽  
pp. 1142-1147 ◽  
Author(s):  
Opender Koul
Keyword(s):  
Biological Activity ◽  
Tribolium Castaneum ◽  
Azadirachta Indica ◽  
Sitophilus Oryzae ◽  
Stored Grain ◽  
Stored Grain Pests

scholarly journals Phytochemical Action of White Mulberry Extracts against Three Important Stored Grain Pests

2017 ◽  
Vol 9 (4) ◽  
pp. 359-366
Author(s):  
Z. R. Sabuj ◽  
A. Khatun ◽  
M. Abdullah ◽  
D. M. Badruddoza ◽  
H. Ali ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Tribolium Castaneum ◽  
Sitophilus Oryzae ◽  
Stem Bark ◽  
Callosobruchus Chinensis ◽  
Ether Extract ◽  
Stored Grain ◽  
Chcl3 Extract ◽  
Stored Grain Pests ◽  
White Mulberry

Petroleum ether (Pet. ether), CHCl3 and CH3OH extracts of white mulberry were subjected to dose-mortality tests against Callosobruchus chinensis (L.), Sitophilus oryzae (L.) and Tribolium castaneum (Hbst.). Pet. ether extract of  leaves against C. chinensis, S. oryzae and T. castaneum provided LD50 9.16, 5.06, 3.79, 2.81, 2.06, 1.55, 1.32, 1.38 mg/g; 17.6, 12.9, 11.3, 82.4, 83.5, 9.47, 8.73, 8.61 mg/g and 0.93, 0.826, 0.691, 0.623, 0.567, 0.532, 0.525 mg/cm2 after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against C. chinensis Pet. ether extract of root and stem bark gave LD50 28.3, 20.7, 18.4, 16.9, 14.4, 13.6, 13.3, 13.3 mg/g and 86.3, 228.9, 177, 109, 61.0, 21.8, 0.999, 15 mg/g after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. CH3OH extracts of leaves offered LD50 21.5, 20.4, 17, 16.2, 15.6, 14.7 mg/g against C. chinensis; 5.4, 14.7, 13.7, 11, 10.9, 9.46, 9.10 mg/g against S. oryzae; and 3.804, 3.429, 3.284, 2.736, 2.441, 2.311, 2.264 mg/cm2 against T. castaneum after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against S. oryzae CH3OH extract of root and CHCl3 of stem bark gave LD50 19.5, 19.1, 17.5, 16.7, 16.2, 15.8, 15.9 mg/g and 9.77, 9.44, 7.71, 6.56, 5.94, 5.39, 5.4 mg/g after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. However, CHCl3 extract of leaves and root didn’t show mortality.

scholarly journals Pathogenicity of Pakistani isolates of Steinernema bifurcatum and S. affine (Rhabditida: Steinernematidae) in management of stored grain pests Lasioderma serricorne and Tribolium castaneum (Coleoptera: Ptinidae, Tenebrionidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Tabassum Ara Khanum ◽  
Salma Javed
Keyword(s):  
Biological Control ◽  
Tribolium Castaneum ◽  
Biological Control Agent ◽  
Economic Loss ◽  
Research Area ◽  
Control Agent ◽  
Lasioderma Serricorne ◽  
Stored Grain ◽  
Insect Infestations ◽  
Stored Grain Pests

Abstract Background Stored grain products suffer a considerable economic loss due to insect infestations. The resistance of pests to insecticide, and residues on crops are produced by the application of insecticide. Nowadays, incremental necessity has been occurred to find out alternatives to chemicals. As biological control agents, entomopathogenic nematodes (EPNs) attract attention increasingly in the research area recently. Results The aim of the study was to evaluate the pathogenicity of Pakistani isolates Steinernema bifurcatum and S. affine against the stored grain pests, Tribolium castaneum and Lasioderma serricorne, under laboratory conditions. Suspensions of nematodes were applied at 4 different concentrations 0, 50, 100, and 200 IJs/ml and 3 variable temperatures 15, 30, and 45°C. Pathogenicity rate was recorded after 48 h of application. The larval stage is found more susceptible than the adult. At 200 IJs, S. bifurcatum showed high mortality of L. serricorne larva (92%) and T. castaneum larva (93%), whereas S. affine showed 90 and 95% mortality of L. serricorne and T. castaneum larva, respectively, at 200 IJs. The results revealed that both species of EPN were able to control and reproduce on L. serricorne and T. castaneum. Maximum mortality was recorded at 45 °C from S. bifurcatum whereas S. affine at 15 °C. Conclusions Therefore, S. bifurcatum and S. affine could be suggested as a biological control agent for hot and cold climatic zones, respectively.

The frequency of Cryptolestes spp. in grain in south-east Queensland

1980 ◽  
Vol 20 (103) ◽  
pp. 234 ◽  
Author(s):  
ER Sinclair ◽  
M Bengston
Keyword(s):  
Sitophilus Oryzae ◽  
Common Species ◽  
Red Flour Beetle ◽  
Rhyzopertha Dominica ◽  
Stored Grain ◽  
Grain Beetles ◽  
Farm Survey ◽  
Stored Grain Pests ◽  
Grain Protectant ◽  
Grain Farms

In a survey of 60 grain farms on the Darling Downs, Queensland, during 1977-78, the flat grain beetles Cryptolestes spp., which are germ feeders in stored grains, were found on 39 of the farms and in 15% of the 483 samples taken. Ninety percent of infestations involving Cryptolestes spp. were found in bag or bulk stores of seed and feed grain, where they were usually associated with heavy infestations of the three more common stored grain pests, the rice weevil Sitophilus oryzae (L.), the lesser grain borer Rhyzopertha dominica (F.), and the red flour beetle Tribolium castaneum (Herbest.). Three species of Cryptolestes were detected: C. pusillus (Schonherr) (45.6% of farms surveyed), C. ferrugineus (Steph.) (28.1 %), and C. pusilloides (Steel and Howe) (15.8%). During the farm survey, samples were also collected from 8 of the 11 central storages reported infested with Cryptolestes spp. C. ferrugineus was present in each of these samples, C. pusillus in only one, whilst C. pusilloides was not found. The three species were tested for resistance to malathion and fenitrothion, the most commonly used grain-protectant chemicals, by exposing adults to treated wheat. C. pusillus and C. pusilloides were susceptible to both malathion and fenitrothion (LC99.9 < 1 mg kg-1). C. ferrugineus, whilst susceptible to fenitrothion (LC99.9 < 1 mg kg-1) was resistant to malathion, with LC99.9 of 300-400 mg kg-1 compared with the recommended dose of 18 mg kg -1. Although C. pusillus was the most common species on farms, it was concluded that C. ferrugineus was the most important overall because it is biologically better suited to the grain in central storages, which is typically hot (>30�C) and of low moisture content (< 12% for wheat).

scholarly journals Molecular characterization and identification of three stored grain pests based on mitochondrial cytochrome C oxidase subunit I (COI) gene sequences

2019 ◽  
Vol 47 (1) ◽  
pp. 1-11
Author(s):  
Abu Faiz Md Aslam ◽  
Sharmin Sultana ◽  
Faria Farhana Rain ◽  
Sumita Rani Das ◽  
Ayesha Siddika ◽  
...  
Keyword(s):  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Dna Barcode ◽  
Control Measures ◽  
Coi Gene ◽  
Composition Analysis ◽  
Callosobruchus Chinensis ◽  
Pest Species ◽  
Stored Grain ◽  
Stored Grain Pests

Stored grain pests are discovered in food as immature stages, which further complicates the identification process. A DNA barcode dataset of some important pests that can be used for easy and confirm identification in stages of life is constructed. COI genes of three stored grain insect pests i.e,, Sitophilus oryzae, Callosobruchus chinensis and Oryzaephilus surinamensis were sequenced. The sequenced genes were submitted to NCBI GenBank and obtained accession numbers MG967331.1, MG967332.1, MG967333.1 and MK041216.1. BLAST analysis showed 99 to 100% homology with existing GenBank sequences. The nucleotide composition analysis revealed that the value of A+T (64.8%) is greater than G+C (35.2%). Genetic distance among four sequences of three store pests were ranged from 0.00293-0.32807. Phylogenetic analysis showed that these three species are originated from different clades. Haplotype analysis of mitochondrial COI gene of the stored grain insect pests showed high genetic diversity among them. C. chinensis, O. surinamensis and S. oryzae were separated from their common ancestor by 80, 73 and 64 mutational steps. These information may be helpful for attempting any successful control measures against the pest species. In conclusion, present author established the first DNA barcode dataset of three store grain pests and confirmed its efficiency for identifying these pests. Bangladesh J. Zool. 47(1): 1-11, 2019

Toxic and repellent action of sugarcane bagasse-based lignin against some stored grain insect pests

1970 ◽  
Vol 25 ◽  
pp. 27-30 ◽  
Author(s):  
LA Muslima Khanam ◽  
D Talukder ◽  
MA Hye
Keyword(s):  
Tribolium Castaneum ◽  
Sugarcane Bagasse ◽  
Laboratory Experiments ◽  
Callosobruchus Maculatus ◽  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Repellent Activity ◽  
Stored Grain ◽  
Time Intervals ◽  
Class V

Laboratory experiments were carried out to investigate the efficacy of sugarcane bagasse-based lignin against four stored grain insect pests viz., Tribolium castaneum, Tribolium confusum, Sitophilus oryzae and Callosobruchus maculatus. Mortality was assessed after 24, 48 and 72 h of treatment and LD50 values were calculated at the respective time intervals. It was observed that T. castaneum is more susceptible to the lignin than the other test insects. Repellent activity of the product was conducted for T. castaneum and T. confusum. All the concentrations of the product were found to be repellent to both the insect species. Strong repellent activity was observed at concentrations of 471.57 and 628.76 µg.cm-2 to T. confusum showing the repellency of class V activity. Key words: Bagasse-based lignin, toxicity, repellency, Tribolium castaneum, T. confusum, Sitophilus oryzae, Callosobruchus maculatus Univ. j. zool. Rajshahi Univ. Vol.25 2006 p.27-30

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